Journal: Annals of Medicine

Article Title: LPI-GPR55 promotes endothelial cell activation and inhibits autophagy through inducing LINC01235 expression

doi: 10.1080/07853890.2024.2407525

Figure Lengend Snippet: LPI targeted GPR55 and promoted endothelial cell activation. (a, b) Western blot analysis of GPR55 and ICAM1 protein levels after treating with LPI (1, 5 and 10 μM) for 18 h. (c, d) RT-PCR analysis of ICAM1 and GPR55 mRNA levels after treating with LPI (1, 5 and 10 μM) for 18 h. (e, g) HAECs were transfected with siGPR55 at 20 and 60 nM for 24 h. RT-PCR and Western blot analysed the RNA and protein level of GPR55. (f) HAECs were transfected with siGPR55 (60 nM) for 24 h and then treated with LPI (10 μM) for 18 h. Western blot analysed ICAM1 protein level. (h) Immunofluorescence analysed the adhesion of monocytes to endothelial cells after treating with LPI (10 μM) for 18 h, scar bar: 500 μm. Data are represented as mean ± SD (* p < .05, ** p < .01 and *** p < .001, n = 3).

Article Snippet: In this study, we used human aortic endothelial cells (HAECs: ATCC ® PCS-100-011, accession no. CVCL_C0EQ) as VEC model. HAECs were grown in vascular cell basal medium (ATCC ® PCS-100-030) with an endothelial cell growth kit-VEGF (ATCC ® PCS-100-041) in a humidified incubator at 37 °C with 5% CO 2 .

Techniques: Activation Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Immunofluorescence

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet: β1IAS functional characterization in genetic β1IAS KI TeloHAECs (A) Western blot showing the expression of β1 integrin and NanoBiT tag in β1IAS knockin (KI) ECs compared to WT TeloHAECs (parental). (B) Relative adhesion measured by the xCELLigence system in β1IAS KI ECs plated on FN compared to parental ECs. Data are the mean ± SD of four independent experiments. Statistical analysis: two-way ANOVA and Bonferroni’s post hoc analysis. (C) Luminescence of β1IAS KI ECs adhering for 30 min to increasing amounts (125–1,000 ng/mL) of FN, collagen type I (Coll I), or laminin 511 (Lam 511). Data are mean ± SD of three independent experiments. Statistical analysis: two-way ANOVA and Bonferroni’s post hoc analysis. (D) Luminescence of β1IAS KI ECs adhering for 30 min to increasing amounts (125–1,000 ng/mL) of vitronectin (VN). Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (E) Luminescence of β1IAS KI ECs adhering for 30 min to FN-coated, increasingly stiff (10 and 100 kPa) PAGEs. Data are mean ± SD of four independent experiments. Statistical analysis: two-tailed heteroscedastic Student’s t-test. (F) Luminescence of β1IAS KI ECs adhering for 15 min to 500 ng/mL FN in the presence of 9EG7 and 12G10 antibodies. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (G) Luminescence of β1IAS KI ECs adhering for 15 min to 500 ng/mL FN in the presence of mAb13 or the pan-αv integrin and α5β1 antagonist MK-0429 (100 μM). Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (H) Luminescence of control β1IAS KI ECs (siCTL) and β1IAS KI ECs silenced for TLN1, FERMT2, and FERMT3 and then plated on 500 ng/mL FN. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (I) Luminescence of control β1IAS KI ECs (siCTL) and β1IAS KI ECs silenced for FLRT2, LPHN2, and PLXND1 and then plated on 500 ng/mL FN. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis.

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: Functional Assay, Western Blot, Expressing, Knock-In, Two Tailed Test, Control

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet: siRNA HTS in β1IAS TeloHAECs to identify activators and inhibitors of β1 integrin (A) Schematic of the high-throughput screening (HTS)-selected genes (194), among the 2,970 genes expressed by parental WT TeloHAECs, whose silencing in β1IAS KI ECs induces a decreased luminescent signal ( Z score < −2), therefore β1 integrin activators (142, in green), and those whose silencing induces an increased luminescent signal ( Z score > 2), therefore β1 integrin inhibitors (52, in red). (B) Bubble plot representing top integrin focused enriched pathways (adjusted p < 0.05) based on candidate genes obtained from the HTS (2 < Z score < −2). All enriched pathways are listed in Table S2 . The EnrichR combined score is the log of the p value from the Fisher exact test multiplied by the Z score of the deviation from the expected rank. Bubble color (adjusted p value) was computed using the Benjamini-Hochberg method for correction for multiple hypotheses testing. The gene ratio is the overlap between the input list and the gene sets in each gene set library for ranking a pathway’s relevance to the input list. (C) Luminescent intensity Z score mean of three biological replicates for each endothelial gene whose siRNA was contained in the Qiagen Druggable Genome v.3 siRNA library. The listed genes (β1 integrin inhibitors in red and β1 integrin activators in green) were chosen for secondary validation. (D) Luminescence of control β1IAS KI ECs (siCTL) and β1IAS KI ECs silenced for RAP1B, TNS3, and RHOJ and then plated on 500 ng/mL FN. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (E) Luminescence of control β1IAS KI ECs (siCTL) and β1IAS KI ECs silenced for RACGAP1, PJA2, and VEGF-B and then plated on 500 ng/mL FN. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis.

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: High Throughput Screening Assay, Biomarker Discovery, Control

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet: The E3 ubiquitin ligase PJA2 promotes kindlin-2 degradation and inhibits β1 integrin activation in ECs (A) Left: western blot showing the expression of PJA2 in WT TeloHAECs after PJA2 silencing and silenced cells transduced with silencing resistant murine PJA2 (mPJA2). Right: luminescence intensity of β1IAS KI ECs plated on 500 ng/mL FN, silenced for PJA2, and rescued with mPJA2. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (B) Western blot showing the expression of kindlin-2, talin-1, and Rap1B in WT TeloHAECs after PJA2 silencing. (C) Left: western blot showing ubiquitinated GFP kindlin-2 pulled down by ubiquitin affinity beads in Phoenix cells overexpressing PJA2 or control construct. The first lane corresponds to the incubation of lysate from cells overexpressing control constructs on non-ubiquitinated beads (see ). Right: western blot showing the expression of PJA2 in cells used in the ubiquitinated assay shown on the left. (D) Confocal microscopy showing 9EG7 + active β1 integrin (green), kindlin-2 (red) and vinculin (blue) in WT TeloHAECs plated on 1.5 μg/mL FN and silenced for PJA2. The image insets highlight focal adhesion sites. Scale bar: 20 μm. (E) Relative maximum Feret diameter (mFD) of adhesion sites (FA) in WT TeloHAECs silenced for PJA2 compared to siCTL. Data are the mean ± SD of three independent experiments (10 cells each). Statistical analysis: two-tailed heteroscedastic Student’s t test. (F) Number of adhesion sites (FA) in siCTL and siPJA2 ECs as in (E). Data are the mean ± SD of three independent experiments (9 cells each). Statistical analysis: two-tailed heteroscedastic Student’s t test.

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: Ubiquitin Proteomics, Activation Assay, Western Blot, Expressing, Transduction, Control, Construct, Incubation, Confocal Microscopy, Two Tailed Test

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet: VEGF-B is an effective inhibitor of β1 integrin activation in ECs (A) Left: western blot showing the expression of VEGF-B protein in WT TeloHAECs after VEGFB silencing compared to siCTL. Right: luminescence of TeloHAEC β1IAS plated on 500 ng/mL FN and silenced for VEGFB in the presence or absence of exogenous VEGF-B for 15 min. Data are mean ± SD of three independent experiments. Statistical analysis: one-way ANOVA and Bonferroni’s post hoc analysis. (B) Confocal microscopy showing 9EG7 + active β1 integrin (green), kindlin-2 (red), and vinculin (blue) in WT TeloHAECs plated on 1.5 μg/mL FN and silenced for VEGFB. The image insets highlight focal adhesion sites. Scale bar: 20 μm. (C) Relative maximum Feret diameter (mFD) of adhesion sites (FA) in WT TeloHAECs silenced for VEGFB compared to siCTL. Data are the mean ± SD of three independent experiments (10 cells each). Statistical analysis: two-tailed heteroscedastic Student’s t test. (D) Number of adhesion sites (FA) in WT TeloHAECs silenced for VEGFB compared to siCTL. Data are the mean ± SD of three independent experiments (9 cells each). Statistical analysis: two-tailed heteroscedastic Student’s t test. (E) Relative adhesion measured by the xCELLigence system in WT TeloHAECs plated on 1.5 μg/mL FN and silenced for VEGFB. Data are the mean ± SD of five independent experiments. Statistical analysis: two-way ANOVA and Bonferroni’s post hoc analysis. (F) Relative adhesion measured by the xCELLigence system in WT TeloHAECs plated on 1.5 μg/mL FN and treated or not with 100 and 200 ng/mL exogenous VEGF-B. Data are the mean ± SD of three independent experiments. Statistical analysis: two-way ANOVA and Bonferroni’s post hoc analysis.

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: Activation Assay, Western Blot, Expressing, Confocal Microscopy, Two Tailed Test

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet: VEGF-B modulates the phosphorylation of CMSC mediators (A and B) Volcano plots of the phosphoproteome of parental WT TeloHAECs stimulated with VEGF-B or control for 15 (A) or 30 (B) min. n = 4 biological replicates. Colored dots are phosphorylation sites of proteins annotated to the GOBP categories “cell adhesion” or “cytoskeleton organization.” Dashed bars separate significantly regulated sites with p ≤ 0.05 and difference ≥ ±0.2. The position of the phosphorylation site within the protein sequence is in parentheses following the gene name. (C and D) Heatmaps of the up- and downregulated sites highlighted in the volcano plots in (A) and (B), respectively. Colors are based on the intensity values measured for the phosphorylated peptide by MaxQuant; purple represents upregulation upon VEGF-B stimulation, and green represents downregulation upon VEGF-B stimulation. An ∗ indicates a known regulatory site. (E) Left: confocal microscopy showing talin-1 (green) and KANK3 (red) in WT TeloHAECs plated on 1.5 μg/mL FN and silenced for VEGFB. The image insets highlight contact sites between talin-1 + adhesions and the CMSC mediator KANK3. Scale bar: 20 μm. Right: Pearson correlation between talin-1 + adhesions and the CMSC mediator KANK3. Data are the mean ± SD of two independent experiments (10 cells each). Statistical analysis: two-tailed heteroscedastic Student’s t test.

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: Phospho-proteomics, Control, Sequencing, Confocal Microscopy, Two Tailed Test

Journal: Cell Reports

Article Title: Luminescent sensing of conformational integrin activation in living cells

doi: 10.1016/j.celrep.2025.115319

Figure Lengend Snippet:

Article Snippet: hTERT-immortalized human aortic endothelial cells (TeloHAECs) , ATCC , CRL-4052.

Techniques: Recombinant, Fluorescence, Ubiquitin Proteomics, Bicinchoninic Acid Protein Assay, Magnetic Beads, Plasmid Preparation, Software

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation

doi: 10.3389/fbioe.2022.903907

Figure Lengend Snippet: Effects of the presence (BM) versus the absence (NBM) of basement membrane proteins on human aortic endothelial cell (hAECs) migration and proliferation. Inverted microscopy images of GFP-labeled hAECs demonstrate that cells seeded on the BM surface cover a larger surface area than those seeded on the non-basement membrane protein (NBM) surface following 20 days in culture (A) . Dashed lines for Day 1 images indicate the initial seeding location of the cells. Dashed lines on Day 20 images indicate the final location of the cells. Due to the uneven surface of the scaffolds and the imaging method (stitch function in microscope Nikon Eclipse Ts2R), different values of cell brightness and focus are present in the images. A quantitative analysis of cell migration distance shows a significant increase in the maximal migration distance for cells seeded on the BM surface versus the NBM surface at all post-seeding timepoints (B) . Analysis of cell migration only (i.e., proliferation inhibited with mitomycin) shows a significant difference in the maximal radial distance migrated by treated-hAECs seeded on the BM surface (BM mitomyocin) when compared to treated-hAECs seeded on the NBM surface (NBM mitomyocin) at all timepoints (C) . Groups not connected by the same lower case letter are significantly different. Maximal migratory distance following 9 days of culture was reduced by the addition of mitomycin for cells seeded on the BM surface, but not for those seeded on the NBM surface (D) Assessment of proliferation only (i.e., FUCCI staining) demonstrated a significantly higher number of proliferating hAECs on the BM surface when compared to the cells in the NBM surface (D) . Scale bar A, 5,000 µm * = p < 0.05, ** = p < 0.01, *** = p < 0.001.

Article Snippet: Human aortic endothelial cells (hAECs), GFP-labeled hAECs (GFP-hAECs), human umbilical vein cells (HUVECs), and GFP-labeled HUVECS (GFP-HUVECs, all cells from Angio-Proteomie, Boston, MA) were expanded to P4, unless otherwise stated, in a T-25 flask coated with quick coating solution (Angio-Proteomie) at 37 ° C, 5% CO 2 .

Techniques: Membrane, Migration, Inverted Microscopy, Labeling, Imaging, Microscopy, Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation

doi: 10.3389/fbioe.2022.903907

Figure Lengend Snippet: Effects of the presence (BM) versus the absence (NBM) of basement membrane proteins on hAEC phenotypes and functions. Representative images of immunofluorescent staining of human laminin production demonstrates the increased production of human laminin in the hAECs seeded on the non-basement membrane protein (NBM) surface when compared to cells seeded on the BM surface (A) . A quantitative analysis of the human laminin production per cell shows a significant higher production of human laminin by the hAECs seeded on the NBM surface versus on the BM surface (D) . Representative images of immunofluorescent staining for endothelial cell polarization demonstrates that the BM presence mediates the polarization of podocalyxin (green) to the apical cell surface of seeded hAECs, indicating their quiescent phenotype (B) . Conversely, apical polarization of podocalyxin is not present on hAECs seeded on the NBM protein side (B) . Similarly, representative images of the adherence junction immunofluorescent staining for VE-cadherin (red) and β-catenin (green) resulted in appropriate expression and co-localization of these adherence junction proteins (indicated by the arrow) in hAECs seeded on the BM surface, while expression and co-localization is not seen in cells seeded on the NBM protein surface (C) . A quantitative analysis of nitrate and nitrite secretions (nitric oxide biproducts) of HUVEC-seeded AR-scaffolds, show a significant increase in nitrate and nitrite secretions by cells seeded on the BM protein surface, compared to the cells seeded on the NBM protein surface or tissue culture plastic (E) . Scale bar A, 100 µm. Scale bars B and C, 10 µm * = p < 0.05, ** = p < 0.01.

Article Snippet: Human aortic endothelial cells (hAECs), GFP-labeled hAECs (GFP-hAECs), human umbilical vein cells (HUVECs), and GFP-labeled HUVECS (GFP-HUVECs, all cells from Angio-Proteomie, Boston, MA) were expanded to P4, unless otherwise stated, in a T-25 flask coated with quick coating solution (Angio-Proteomie) at 37 ° C, 5% CO 2 .

Techniques: Membrane, Staining, Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation

doi: 10.3389/fbioe.2022.903907

Figure Lengend Snippet: Differential gene expression and the gene set enrichment analysis of scaffold seeded, simvastatin-, and TNFα-treated hAECs. (A) Heatmap of all 415 identified significantly differentially expressed genes across all three treatment groups. (B–D) Significantly enriched pathways in the KEGG and HALLMARK gene sets following GSEA for simvastatin (B) , scaffold (C) , and TNFα (D) groups. (E–G) Representative GSEA plots across all three groups for three biologically relevant pathways, including mitosis (E) , programmed cell death (F) , and glucose metabolism (G) .

Article Snippet: Human aortic endothelial cells (hAECs), GFP-labeled hAECs (GFP-hAECs), human umbilical vein cells (HUVECs), and GFP-labeled HUVECS (GFP-HUVECs, all cells from Angio-Proteomie, Boston, MA) were expanded to P4, unless otherwise stated, in a T-25 flask coated with quick coating solution (Angio-Proteomie) at 37 ° C, 5% CO 2 .

Techniques: Gene Expression

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation

doi: 10.3389/fbioe.2022.903907

Figure Lengend Snippet: tSNE clustering analysis of scaffold seeded, simvastatin, and TNFα-treated hAECs. The tSNE clustering analysis plot is driven by the top 1,000 variable genes across all samples (A) . PC1 accounts for 79.88% of the variance, while PC2 explains the remaining 20.22%. Heatmap of the top 100 genes responsible for driving PC1 (B) . PC1 is almost exclusively driven by up-regulated genes in the TNFα group. Enrichment plot of the top 10 enriched gene ontology biological processes for the top 100 genes driving PC1 (D) . The top 10 enriched pathways in PC1 are all associated with pro-inflammatory responses. Heatmap of the top 100 genes responsible for driving PC2 (C) . PC2 is predominantly driven by up-regulated genes in the scaffold group. Enrichment plot of the top 10 enriched gene ontology biological processes for the top 100 genes driving PC2 (E) . 50% of the enriched pathways in PC2 are associated with cellular chemotaxis and respond to chemokines.

Article Snippet: Human aortic endothelial cells (hAECs), GFP-labeled hAECs (GFP-hAECs), human umbilical vein cells (HUVECs), and GFP-labeled HUVECS (GFP-HUVECs, all cells from Angio-Proteomie, Boston, MA) were expanded to P4, unless otherwise stated, in a T-25 flask coated with quick coating solution (Angio-Proteomie) at 37 ° C, 5% CO 2 .

Techniques: Chemotaxis Assay

Journal: Scientific Reports

Article Title: Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

doi: 10.1038/s41598-019-53230-1

Figure Lengend Snippet: ECM niche modulates differential morphology of seeded hAEC. ( A – D ) Representative images of eGFP-hAEC seeded on fibrous or serous side of 6 mm AR-BP discs and imaged after 2 ( A , C ) and 4 ( B , D ) days show that higher number of cells can be observed on serous side compare to fibrous. ( E – H ) When seeded at higher densities and cultured for 2 days, eGFP-hAEC (( E ) 71 cells/mm 2 ; ( F ) 142 cells/mm 2 ; ( G ) 284 cells/mm 2 , ( H ) 568 cells/mm 2 ) seeded on the serous side show multiple vacuoles (arrows) and rounded morphology compared to fibrous side. ( I ) Quantification of cell aspect ratio demonstrates that cells seeded on serous side are statistically more rounded (lower aspect ratio) than those seeded on the fibrous side, and that cell morphology becomes increasingly more rounded with higher seeding densities regardless of seeding side. Scale bar 1 mm ( A , B ), 200 μm ( C – H ). Data represent the mean values natural log-transformed aspect ratios ± SD, n = 5 per group per seeding density (****p < 0.0001).

Article Snippet: Enhanced green fluorescent protein (eGFP) transfected hAEC were purchased from Angio-Proteomie (Boston, MA) (CAT No. cAP‐0006GFP, LOT No. 201209701SR).

Techniques: Cell Culture, Transformation Assay

Journal: Scientific Reports

Article Title: Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

doi: 10.1038/s41598-019-53230-1

Figure Lengend Snippet: Actin expression in eGFP-hAEC seeded on AR-BP scaffolds. Representative microscopic images (n = 5 per group) of eGFP-hAEC (green, first column) after 4 days proliferation and staining for Actin (Phaliodin-594; red, second column) and nuclei (Hoechst 33342; blue, third column). ( A , B ) 71 cell/mm 2 ; ( C , D ) 142 cell/mm 2 ; ( E , F ) 284 cell/mm 2 and ( G , H ) 568 cell/mm 2 . Scale bars: 100 μm (first to fourth column) and 20 μm (fifth column). ( I ) Quantification of cell number on the serous and the fibrous side 4 days after seeding at different densities demonstrate higher cell numbers in the serous side for low and moderate seeding densities (71, 142, and 284 cells/mm 2 ), while cell number equalize when seeded at very high densities (568 cells/mm 2 ). Data represent the average number of cells in a 1.6 mm 2 area ± SD, n = 5 images per group (seeding density and side) (**p < 0.01, *p < 0.05, n.s. non-significant p = 0.4).

Article Snippet: Enhanced green fluorescent protein (eGFP) transfected hAEC were purchased from Angio-Proteomie (Boston, MA) (CAT No. cAP‐0006GFP, LOT No. 201209701SR).

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

doi: 10.1038/s41598-019-53230-1

Figure Lengend Snippet: ECM niche modulates hAEC human laminin production following seeding on AR-BP scaffolds. Representative fluorescent microscopy images of ( A’ – D’ ) cell nuclei (blue), ( A” – D” ) eGFP-hAEC (green), and ( A”’ – D”’ ) human laminin (red). The serous side was found to have a continuous laminin layer while on the fibrous side new laminin production was found to be strongly associated with areas of cellular adhesion, especially at low seeding densities (n = 5 per group). ( E , F ) Quantification of human laminin production per cell. At moderate seeding density (142 cells/mm 2 ) hAEC seeded on the serous side produce more laminin per cell than those seeded on the fibrous side (p = 0.0002). At high seeding density (568 cells/mm 2 ) the effect of ECM niche on per cell laminin production is no longer present. Scale bar = 10 µm.

Article Snippet: Enhanced green fluorescent protein (eGFP) transfected hAEC were purchased from Angio-Proteomie (Boston, MA) (CAT No. cAP‐0006GFP, LOT No. 201209701SR).

Techniques: Microscopy